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The effect of dietary protein quality on free amino acids in plasma, muscle and liver of growing chickens

  • I. Fernández-Figares (a1), M. Lachica (a1), L. Pérez (a1), R. Nieto (a1), J. F. Aguilera (a1) and C. Prieto (a1)...

Abstract

Free amino acid (AA) levels in plasma, muscle and liver were measured in growing chickens given either high or low protein diets varying in quality. In experiment 1, they were force-fed once a day (09.00 h), for 4 days, at about 1·5 × M level, a nitrogen-free (NF) diet and then, on day 5, they were given either diet NF or isoenergetic (13·1 kj metabolizable energy (ME) per g dry matter (DM)) and isonitrogenous high protein diets (200 g crude protein (CP) per kg) based on casein (C), lupin (L), soya bean (SB), faba bean (FB), field pea (FP), vetch (V) or bitter vetch (B) as the sole source of protein. In experiment 2, chickens were force-fed twice a day (09.00 h and 18.00 h), for 3 days, at about 1·9 × M level, with four isoenergetic (13·1 k) ME per kg DM) and isonitrogenous low protein diets (120 g CP per kg) based on SB, FP, V or B as the sole source of protein. On days 5 (experiment 1) and 4 (experiment 2) samples of plasma, muscle and liver were taken for AA analysis over 3 to 4h after morning meal.

In general, within experiments, no significant differences in AA concentrations in plasma, muscle or liver among diets were found. However, there was a qualitative but not a quantitative agreement between the AA abundance in tissues and the AA rank of dietary protein. Moreover, when pooling data from experiments 1 and 2, significant regressions were found between the levels of threonine, aspartic acid, glutamic acid, glycine and proline in plasma, of lysine, alanine, glutamic acid, glycine and proline in muscle or that of proline in liver and the corresponding amounts ingested with the different diets. Under the conditions of these experiments, however, it was not possible to establish conclusively a direct relationship between the level of free amino acids in tissues and dietary protein quality.

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Aguilera, J. F. and Prieto, C. 1987. [Energy requirement for maintenance in growing chickens.] Archivos de Zootecnia 36: 165172.
Almquist, H. J. 1954. Utilization of amino acids by chicks. Archives of Biochemistry 52:197202.
Bergen, W. G. and Purser, D. B. 1968. Effect of feeding different protein sources on plasma and gut amino acids in the growing rat. Journal of Nutrition 95: 333340.
Bodwell, C. E. 1975. Biochemical parameters as indices of protein nutritional value. In Protein nutritional quality of foods and feeds (ed. Friedman, M.), part 1, pp. 261310. Marcel Decker, New York.
Cohen, S. A., Meys, M. and Tarvin, T. L. 1989. The Pico-Tag Method. A manual of advanced techniques for amino acid analysis. Millipore Corporation. Bedford, Massachusetts.
Eggum, B. O. 1976. Indirect measures of protein adequacy. In Protein metabolism and nutrition (ed. Cole, D. J. A., Boorman, K. N., Buttery, P. J., Lewis, D., Neale, R. J. and Swan, H.), pp. 249258. Butterworths, London.
Feigin, R., Beisel, W. R. and Wannemacher, R. W. 1971. Rhythmicity of plasma amino acids and relation to dietary intake. American journal of Clinical Nutrition 24: 329341.
Fugita, Y., Yamamoto, T., Rikimaru, T., Ebisawa, H. and Inoue, G. 1981. Effect of quality and quantity of dietary protein on free amino acids in plasma and tissues of adult rats. Journal of Nutritional Science and Vitaminology 27: 129147.
Galibois, I., Parent, G. and Savoie, L. 1987. Effect of dietary proteins on time-dependent changes in plasma amino acid levels and on liver protein synthesis in rats. Journal of Nutrition 117: 20272035.
Hagemeister, H., Scholz-Ahrens, K. E., Schulte-Coerne, H. and Barth, C. A. 1990. Plasma amino acids and cholesterol following consumption of dietary casein or soy protein in minipigs. Journal of Nutrition 120:13051311.
Hill, D. C. and Olsen, E. M. 1963. Effect of starvation and a nonprotein diet on blood plasma amino acids, and observations on the detection of amino acids limiting growth of chicks fed purified diets. Journal of Nutrition 79: 303310.
Jansen, G. R., Schibly, M. B., Masor, M., Sampson, D. A. and Longenecker, J. B. 1986. Free amino acid levels during lactation in rats: effects of protein quality and protein quantity. Journal of Nutrition 116: 376387.
Johnson, D. and Anderson, H. 1982. Prediction of plasma amino acid concentration from diet amino acid content. American Journal of Physiology 243: R99–R103.
Kunachowicz, H., Klys, W., Czarnowska-Misztal, E. 1983. Effect of dietary protein quantity and quality on free amino acids levels in the serum and muscles of experimental rats. Proceedings of fourth international symposium on protein metabolism and nutrition, les colloques de I'INRA, n° 16, pp. 117120. Clermont Ferrand, France.
Longenecker, J. B. and Hause, N. L. 1959. Relationship between plasma amino acids and composition of the ingested protein. Archives of Biochemistry and Biophysics 84: 4659.
Moore, S. 1963. On the determination of cystine as cysteic acid. Journal Biological Chemistry 238: 235237.
Munro, H. N. 1970. Free amino acid pools and their role in regulation. In Mammalian protein metabolism (ed. Munro, H. N.), vol. IV, pp. 299386. Academic Press, New York.
National Research Council. 1984. Nutrient requirements of poultry. National Academy Press, Washington.
Pérez, L., Fernández-Figares, I., Nieto, R., Aguilera, J. F. and Prieto, C. 1993. Amino acid ileal digestibility of some grain legume seeds in growing chickens. Animal Production 56: 261267.
Patureau-Mirand, P., Toullec, R., Paruelle, J. L., Prugnaud, J. and Pion, R. 1974. Influence de la nature des matieres azotées des aliments d'allaitement sur l'aminoacidémie du veau préruminant. Annales de Zootechnie 23: 343358.
Pawlak, M. and Pion, R. 1968. Influence de la supplementation des protéines de blé par des doses croissantes de lysine sur la teneur en acides aminés libres du sang et du muscle du rat en croissance. Annales de Biologic Animate, Biochimie, Biophysique 8: 517530.
Pion, R. 1973. The relationship between the levels of free amino acids in blood and muscle and the nutritive value of proteins. In Protein in human nutrition (ed. Porter, J. W. G. and Rolls, B. A.). Academic Press, New York and London.
Pion, R. 1976. Dietary effects and amino acids in tissues. In Protein metabolism and nutrition (ed. Cole, D. J. A., Boorman, K. N., Buttery, P. J., Lewis, D., Neale, R. J. and Swan, H.), pp. 259277. Butterworths, London.
Prieto, C., Aguilera, J. F., Lachica, M., Fernández-Figares, I., Pérez, L., Nieto, R. and Ferrando, G. 1993. The use of plasma free amino acids for predicting the limiting amino acid(s) in diets for chickens. Animal Feed Science and Technology In press.
Rérat, A., Jung, L. and Kandé, J. 1988. Absorption kinetics of dietary hydrolysis products in conscious pigs given diets with different amounts of fish protein. 2. Individual amino acids. British Journal of Nutrition 60:105120.
Riis, P. M. ed. 1983. Dynamic biochemistry of animal production. Elsevier, Amsterdam.
Statistical Analysis Systems Institute. 1985. SAS procedures guide for personal computers, version 6 edition. SAS Institute Inc., Cary, NC.
Tasaki, I. and Ohno, T. 1971. Effect of dietary protein level on plasma free amino acids in the chicken. Journal of Nutrition 101:12251232.
Waterlow, J. C. and Fern, E. B. 1981. Free amino acid pools and their regulation. In Nitrogen metabolism in man (ed. Waterlow, J. C. and Stephen, J. M. L.), pp. 116. Applied Science Publishing, London.
Zimmerman, R. A. and Scott, H. M. 1965. Interrelationship of plasma amino acid levels and weight gain in the chick as influenced by suboptimal and superoptimal dietary concentrations of single amino acids. Journal of Nutrition 87: 1318.
Zimmerman, R. A. and Scott, H. M. 1967. Effect of fasting and of feeding a nonprotein diet on plasma amino acid levels in the chick. Journal of Nutrition 91: 507508.

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The effect of dietary protein quality on free amino acids in plasma, muscle and liver of growing chickens

  • I. Fernández-Figares (a1), M. Lachica (a1), L. Pérez (a1), R. Nieto (a1), J. F. Aguilera (a1) and C. Prieto (a1)...

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