Skip to main content Accessibility help
×
Home

Blood and tissue lipid composition and lipase activity in wether lambs treated with trenbolone acetate combined with oestradiol-17 β at two different live weights

  • S. B. Singh (a1), J. R. Scaife (a1) and H. Galbraith (a1)

Abstract

Thirty-two Border Leicester ♂ × Scottish Blackface ♀ wether lambs aged about 5 months, were divided on the basis of live weight such that group G1 contained the 16 lightest lambs and group G2 the 16 heaviest lambs. Lambs in group G1 were subdivided equally at random to be either sham-implanted controls (group C1) or to be implanted with 35 mg trenbolone acetate (TBA) + 5 mg oestradiol-17β (OE) (group T1) at 24 kg initial live weight. Lambs in group G2 were also subdivided into two groups (C2 and T2) and similarly treated approximately 1 month later at 37 kg initial live weight. Animals were offered ad libitum, a diet containing an estimated 12·5 MJ metabolizable energy and 140 g crude protein per kg dry matter. The experimental treatments lasted for 60 days.

Samples of pre-heparin plasma were analysed for free fatty acid (FFA) and triglycerides (TG). Post-heparin plasma was analysed for lipoprotein lipase (LPL) activity. Samples of subcutaneous (SCAT), perinephric (PNAT), mesenteric (MAT) and intermuscular (IMAT) adipose tissue, liver and muscle, taken immediately post mortem, were analysed for total lipid concentration and fatty acid composition. Comparisons were made for the main effects of hormonal treatment and live weight.

Plasma FFA concentrations in heavier lambs (group G2) were significantly higher than in group G1 from week 1 pre-implantation to week 3 post implantation. Compared with group G1, animals in group G2 had significantly higher plasma TG concentrations at weeks 1, 2, 5 and 8 when data was analysed using pre-implantation values as covariates. The lipid concentration was lower in group G1 than in group G2. In group G1 compared with group G2 there were greater proportions of C12:0 and C18:2 in SCAT and C16:1 in PNAT and lesser proportions of C18:0 in SCAT and PNAT.

Significant effects due to hormonal treatment were recorded for plasma TG at weeks 1, 5, 6, 7 and 8 mainly due to increases in group T1 compared with group C1. Hormonal treatment increased the proportions of C12:0, C15:0, C16:1 and C18:1 in SCAT and C16:1 and C18:1 in IMAT; reduced the proportions of C16:0 and C18:0 in SCAT and C18:0 in MAT and IMAT and reduced the ratio of saturated to unsaturated fatty acids in SCAT and IMAT and to a lesser extent in MAT.

Copyright

References

Hide All
Abou Akkada, A. R. and El-Shazley, K. 1976. Application of synthetic oestrogens in sheep. In Anabolic Agents in Animal Production (ed. Coulston, F. and Korte, F.), pp. 98108. George Thieme, Stuttgart.
Alderson, E. M., Scaifr, J. R. and Galbraith, H. 1988. The effect of oestradiol-17β and Cimaterol given alone or in combination on lipid metabolism and cAMP concentrations in tissues of male castrate sheep. Proceedings of the Nutrition Society In Press.
Baird, J., Black, N. W. and Faulkner, D. E. 1967. Semi-automated method for the estimation of free fatty acids in plasma. Journal of Clinical Pathology 20: 905909.
Barker, M. E., Scaiee, J. R. and Galbraith, H. 1983. The effect of trenbolone acetate on plasma lipid concentration in mature female sheep. Proceedings of the Nutrition Society 42: 55A (Abstr.).
Bell, A. W. 1979. Lipid metabolism in liver and selected tissues and in the whole body of ruminant animals. Progress in Lipid Research 18: 117164.
Burch, L., Scaife, J. R. and Galbraith, H. 1982. Effect of anabolic steroids on lipogenic and lipolytic enzymes in sheep tissues. Hormone and Metabolic Research 14: 5253.
Busboom, J. J., Miller, G. J., Field, R. A., Crouse, J. D., Riley, M. L., Nelms, G. E. and Ferrell, C. L. 1981. Characteristics of fat from heavy ram and wether lambs. Journal of Animal Science 52: 8392.
Christie, W. W. 1973. Lipid Analysis. Pergamon Press.
Coelho, J. F. S., Galbraith, H. and Topps, J. H. 1981. The effect of a combination of trenbolone acetate and oestradiol-17β on growth performance and blood, carcass and body characteristics of wether lambs. Animal Production 32: 261266.
Crouse, J. D., Kemp, J. D., Fox, J. D., Ely, D. G. and Moody, W. G. 1972. Effect of castration, testosterone and slaughter weight on fatty acid content of ovine adipose tissue. Journal of Animal Science 34: 384387.
Department of Health and Social Security. 1984. Diet and Cardiovascular Disease, Committee on Medical Aspects of Food Policy. Her Majesty's Stationery Office, London.
Galbraith, H. and Topps, J. H. 1981. Effects of hormones on the growth and body composition of animals. Nutrition Abstracts and Reviews, Series B 51: 521540.
Galbraith, H. and Watson, H. B. 1978. Performance, blood and carcase characteristics of finishing steers treated with trenbolone acetate and hexoestrol. Veterinary Record 103: 2831.
Hammond, J. 1932. Growth and the Development of Mutton Qualities in the Sheep. 2nd ed. Oliver and Boyd, Edinburgh.
Hamosh, M. and Hamosh, P. 1975. The effect of oestrogen on the lipoprotein lipase activity of rat adipose tissue. Journal of Clinical Investigation 55: 11321135.
Haugebak, C. D., Hedrick, H. B. and Asplund, J. M. 1974. Relationship between extramuscular adipose tissue lipoprotein lipase activity and intramuscular lipid deposition in fattening lambs. Journal of Animal Science 39: 10261031.
Jacobs, J. A., Field, R. A., Botkin, M. P., Kaltenbach, C. C. and Riley, M. L. 1972. Effect of testosterone enanthate on lamb carcass composition and quality. Journal of Animal Science 34: 3036.
Krauss, R. M., Levy, R. I. and Fredrickson, D. S. 1974. Selective measurement of two lipase activities in postheparin plasma of normal subjects and patients with hyperlipoproteinemia. Journal of Clinical Investigation 54: 11071124.
Laarveld, B., Delorme, B. and Kerr, D. E. 1982. Lipotropic effect of estradiol-17β in fed and fasted lactating cows. Journal of Dairy Science 65: 920926.
Lawes Agricultural Trust. 1977. Genstat V, Mark 4.01. Rothamsted Experimental Station, Harpenden.
MacVinish, L. J. and Galbraith, H. 1988. The effect of implantation of trenbolone acetate and oestradiol-17β in wether lambs at two initial live weights on concentrations of steroidal residues and blood glucose, urea and thyroid hormones. Animal Production 47: 7585.
Prior, R. L., Smith, S. B., Schanbacher, B. D. and Mersmann, H. J. 1983. Lipid metabolism in finishing bulls and steers implanted with oestradiol-17β-dipropionate. Animal Production 37: 8188.
Robelin, J. 1981. Cellularity of bovine adipose tissues: developmental changes from 15–65 percent mature weight. Journal of Lipid Research 22: 452457.
Scaife, J. R., Shehab-Eldin, F. M. and Galbraith, H. 1982. Effects of subcutaneous implantation of trenbolone acetate and oestradiol-17β on plasma lipid concentrations in sheep. Hormone and Metabolic Research 14: 589592.
Sharp, G. D. and Dyer, I. A. 1971. Effects of zearalanol on the performance and carcass composition of growing-finishing ruminants. Journal of Animal Science 33: 865871.
Singh, S. B., Scaife, J. R., Galbraith, H. and Jessiman, C. 1985. Influence of oestrogenic and androgenic compounds on blood lipids, subcutaneous fatty acid composition and activities of certain lipogenic enzymes in sheep. Proceedings of the Nutrition Society 44: 95A (Abstr.).
Sulieman, A. H., Galbraith, H. and Topps, J. H. 1983. Response of mature female sheep to trenbolone acetate. Proceedings of the Nutrition Society 42: 56A (Abstr.).
Sulieman, A. H., Galbraith, H. and Topps, J. H. 1986. Growth performance and body composition of early weaned wether lambs treated with trenbolone acetate combined with oestradiol-17β. Animal Production 43: 109114.
Sulieman, A. H., Galbraith, H. and Topps, J. H. 1988. Growth performance and body composition of wether lambs implanted at two different initial live weights with trenbolone acetate combined with oestradiol-17β. Animal Production 47: 6574.
Tichenor, D. A., Kemp, J. D., Fox, J. D., Moody, W. G. and Deweese, W. 1970. Effect of slaughter weight and castration on ovine adipose fatty acids. Journal of Animal Science 31: 671675.
Trout, D. L., Estes, E. H. and Freidberg, S. J. 1960. Titration of FFA in plasma: A study of current methods and a new modification. Journal of Lipid Research 1: 199202.
Vandermeerschen-Doize, F., Bouchat, J. C., Bouckoms-Vandermeir, M.-A. and Paouay, R. 1983. Effects of long-term ad libitum feeding on plasma lipid components and blood glucose, β-hydroxybutyrate and insulin concentrations in lean adult sheep. Reproduction Nutrition and Developpement 23: 5163.
Vandermeerschen-Doize, F., Bouchat, J. C., Bouckoms-Vandkrmeir, M.-A. and Paouay, R. 1984. Influence of the state of fatness on body composition and blood constituents (lipids, glucose, ketone bodies and insulin) in adult sheep. Zeitschrift fur Tierphysiologie, Tierernährung und Futtermittelkunde 52: 112118.
Watkins, M. L., Fizette, N. and Heimberg, M. 1972. Sexual influences on hepatic secretion of triglycerides. Biochimica Biophysica Acta 280: 8285.
Wood, J. D. 1984. Fat deposition and the quality of fat tissue in meat animals. In Fats in Animal Nutrition (ed. Wiseman, J.), pp. 407435. Butterworths, London.

Related content

Powered by UNSILO

Blood and tissue lipid composition and lipase activity in wether lambs treated with trenbolone acetate combined with oestradiol-17 β at two different live weights

  • S. B. Singh (a1), J. R. Scaife (a1) and H. Galbraith (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.