Skip to main content Accessibility help
×
Home

Adipocyte size and lipogenic enzyme activities in different adipose tissue depots in steers of local Spanish breeds

  • J. A. Mendizabal (a1), P. Albertí (a2), P. Eguinoa (a1), A. Arana (a1), B. Soret (a1) and A. Purroy (a1)...

Abstract

The effect of genotype on the size of adipocytes and on the lipogenic enzyme activities in different fat depots in Asturiana, Avilena, Morucha, Parda Alpina, Pirenaica, Retinta and Rubia Gallega steers was studied (six animals per breed). Steers were weaned at about 6 to 8 months of age and 220 to 260 kg live weight (LW). During the fattening period the steers were given commercial concentrates and straw, both ad libitum, until slaughter at 470 kg average LW. Adipocyte size and activity of the following lipogenic enzymes were studied: glycerol 3-phosphate dehydrogenase (G 3-PDH); fatty acid synthase (FAS); NADP-malate dehydrogenase (MD), glucose 6-phosphate dehydrogenase (G 6-PDH) and NADP-isocitrate dehydrogenase (ICDH) in the omental, perirenal, subcutaneous and intermuscular adipose depots. Also the perirenal fat depot was removed and weighed and the 10th rib was dissected. Differences between breeds were significant for the amount of fat in the different adipose depots (P < 0·001), for adipocyte size (except in intermuscular depot) and for G 3-PDH, FAS, MD, ICDH (P < 0·001) and G 6-PDH (P < 0·01). In general, steers of breeds that had more fat also had bigger adipocytes and higher lipogenic enzyme activities. In this way, Asturiana breed (lean breed) had smaller adipocytes and lower lipogenic enzyme activities than Morucha breed (fatter breed). Finally, adipocytes from the perirenal depot were the biggest and showed the highest G 3-PDH and FAS activities while adipocytes from the intermuscular depot had the smallest size and the lowest lipogenic activities.

Copyright

References

Hide All
Biocom. 1992. Photometric image analysis system, p. 219. Les Ulis Cedex, France.
Cianzio, D. S., Topel, D. G., Whitehurst, G. B. and Beitz, D. C. 1985. Adipose tissue growth and cellularity: changes in bovine adipocyte size and number. Journal of Animal Science 60: 970976.
Glock, G. E. and McLean, P. 1953. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochemical Journal 55: 400408.
Halestrap, A. P. and Denton, R. M. 1973. Insulin and the regulation of adipose tissue acetyl coenzyme A carboxilase Biochemical Journal 105: 529536.
Hood, R. L. 1982. Relationships among growth, adipose cell size, and lipid metabolism in ruminant adipose tissue. Federation Proceedings 41: 25552561.
Hood, R. L. and Allen, C. E. 1973. Cellularity of bovine adipose tissue. Journal of Lipid Research 14: 605610.
Hood, R. L. and Allen, C. E. 1975. Bovine lipogenesis: effects of anatomical location, breed and adipose cell size. International Journal of Biochemistry 6: 121131.
Kempster, A. J. 1980-81. Fat partition and distribution the carcass of cattle, sheep and pigs: a review. Meat Seien 5: 8398.
La Hoz, L. de and Vernon, R. G. 1993. Endocrine control sheep adipose tissue fatty acid synthesis: depot specii differences in response to lactation. Hormone and Metabo< Research 25: 214218.
Mendizabal, J. A., Soret, B., Purroy, A., Arana, A. and Horcada, A. 1997. Influence of sex on cellularity ar lipogenic enzymes of Spanish lamb breeds (Lacha and Ra: Aragonesa). Animal Science 64: 283289.
Ministerio de Agricultura, Pesca y Alimentación. 1986 [Catalogue of Spanish local breeds. 2. Cows], p. 234. Minister de Agricultura, Pesca y Alimentación, Madrid.
Ochoa, S. 1955. Malic enzyme. In Methods in enzymolog volume 1 (ed. Colowick, S. P. and Kaplan, N. O.), pp. 73974 Academic Press, New York.
Plaut, G. W. E. 1962. Isocitric dehydrogenase (TPN-linke< from pig heart (revised procedure). In Methods enzymology, volume 5 (ed. Colowick, S. P. and Kaplar, N. O. p. 645. Academic Press, New York.
Robdell, M. 1964. Metabolism of isolated fat cells. I. Effec of hormones on glucose metabolism and lipolysis. Journal Biological Chemistry 239: 375386.
Robelin, J. 1981. Cellularity of bovine adipose tissue developmental changes from 15 to 65 percent matui weight. Journal of Lipid Research 22: 452457.
Robelin, J. 1986. Growth of adipose tissues in catti partitioning between depots, chemical composition ar cellularity. A review. Livestock Production Science 1 349364.
Rule, D. C., Beitz, D. C. and Hood, R. L. 1987. A note с the effect of adipocyte size on in vitro lipogenesis fro] acetate and lactate in subcutaneous adipose tissue of largì and small-frame beef steers at 6 months of age. Anim Production 44: 454456.
Scott, R. A. and Prior, R. L. 1980. Effects of dietary energ and biological type on lipogenic-related enzymes in be» steers. Journal of Animal Science 50: 137144.
Shidu, K. S., Emery, R. S., Parr, A. F. and Merkel, R. I 1973. Glyceride synthesis in ovine adipose homogenates. Journal of Animal Science 37: 271272.
Statistical Analysis Systems Institute. 1989. SAS/STA user’s guide, release 6.04 edition. Statistical Analysis Systerr Institute, Inc., Cary, NC.
Truscott, T. G., Wood, J. D. and Denny, H. R. 1983. Fat deposition in Hereford and Friesian steers. 2. Cellule development. Journal of Agricultural Science, Cambridge 101 271276.
Vernon, R. G. 1986. The growth and metabolism с adipocytes. In Control and manipulation of animal growth (ed P. J.Buttery, , N. B., Haynes and Lindsay, D. B.). pp. 678. Butterworths, London.
Wisse, L. S. and Green, H. 1979. Participation of on isozyme of cytosolic glycerophosphate dehydrogenase i the adipose conversion of 3T3 cells. Journal of Biologia Chemistry 254: 273275.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed