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Use of Single- and Multi-Locus and Polymerase Chain Reaction Systems for Zygosity Determination - Clinical Application in Twins with Clefts of the Lip and Palate

Published online by Cambridge University Press:  01 August 2014

H. Eufinger ,
S.P. Rand  and
U. Schütte
H. Eufinger*
Affiliation:
University Hospital of Oral & Maxillofacial Surgery - Regional Plastic Surgery, Knappschafts Hospital, Ruhr-University, Bochum, Germany
S.P. Rand
Affiliation:
Institute of Forensic Medicine, Westfälische Wilhelms-University, Münster, Germany
U. Schütte
Affiliation:
Institute of Forensic Medicine, Westfälische Wilhelms-University, Münster, Germany
*
Universitätsklinik f. Mund-, Kiefer- und Gesichtschirurgie - Plastische Operationen -, In der Schornau 23-25, 44892 Bochum, Germany

Abstract

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Precision of zygosity determination in twins can be improved by the use of modern methods of DNA analysis. The clinical application of 4 single- (SLS) and 2 multi-locus (MLS), and 6 PCR (polymerase chain reaction) systems for zygosity determination in 12 twin pairs with oral clefts was compared with regard to the quality and quantity of sample material required and the probability of error in monozygosity determination. PCR systems proved to be superior to SLS or MLS, as DNA sampling is much more convenient, while its level of accuracy still fulfils clinical requirements. For this reason, PCR systems should be considered a basic method in modern clinical twin research.

Keywords

Cleft lip and palateZygosity determinationDNA fingerprintRestriction fragment length polymorphismPolymerase chain reaction
Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 44 , Issue 1 , January 1995 , pp. 25 - 30
Copyright
Copyright © The International Society for Twin Studies 1995

References

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