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Use of Multiple Standards for Absorption Correction and Quantitation with Frieda

Published online by Cambridge University Press:  06 March 2019

P. S. Ong ,
E. L. Cheng  and
G. Sroka
P. S. Ong
Affiliation:
The University of Texas System Cancer Center, M. D., Anderson Hospital and Tumor Institute, Houston, Texas
E. L. Cheng
Affiliation:
The University of Texas System Cancer Center, M. D., Anderson Hospital and Tumor Institute, Houston, Texas
G. Sroka
Affiliation:
The University of Texas System Cancer Center, M. D., Anderson Hospital and Tumor Institute, Houston, Texas
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Abstract

The computerized fluorescence radiation induced energy dispersive analyzer (FRIEDA) (1) described earlier uses an x-ray beam with a well defined energy for the excitation of fluorescence radiation, and an Si(Li) detector to measure the total x-ray spectra emitted. Such a system can also simultaneously provide supplemental data for the determination of the dry mass and the sample mass absorption which is necessary for accurate quantitation of the results. This instrumental capabillty has been utilized in the measurement of the trace elements iron, copper, and zinc in serum.

Known amounts of two elements are thoroughly mixed with the sample. One element has a ‘high energy’ K line, the other a ‘low energy’ K line. The ratio of these intensities, in the absence of absorption, is a known constant and dependent only on the relative amounts of the respective elements, and on the energy of the exciting radiation. Whenever absorption is present, the ratio will change in a manner directly related to the mass absorption of the sample for these radiations.

Type
Research Article
Information
Advances in X-Ray Analysis , Volume 17: Twenty-Second Annual Conference on Applications of X-ray Analysis, August 22-24, 1973 , 1973 , pp. 269 - 278
Copyright
Copyright © International Centre for Diffraction Data 1973

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References

1. Ong, P.S., Lund, P. K., Litton, C. E., and Mitchell, B. A., An Energy Dispersive System for the Analysis of Trace Elements in Human Blood Serum. Advances in X-ray Analysis, Vol. 16, Proc. 21st Ann. Conf. on Application of X-ray Analysis, Denver, 1972.Google Scholar
2. Zelts, L., X-ray Emission Analysis in Biological Specimen. In Progress in Analytical Chemistry, Vol. 3 (X-ray and Electron Probe Analysis in Biomedical Research). Earle, K. M. and Tousimis, A. J., Eds. Plenum Press, N.Y., 1969.Google Scholar
3. Bowen, H. J. M., The Elementary Composition of Mammalian Blood, United Kingdom Atomic Energy Authority Research Group, Report, 1963.Google Scholar