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Composition of Coal Dusts and Their Cytotoxicity on Alveolar Macrophages

  • C. Y. Lee (a1), S.L Lee (a2), C. E. Sheehan (a1) and Yinghua Wang (a1)


Coal mine dust is produced from complex materials which consist of organic sedimentary phases, inorganic minerals, and trace elements. The dust varies in its chemical compositions and is capable of causing lung injury when inhaled. The purpose of this study was to perform scanning electron microscopy combined with energy dispersive spectrometry, wavelength dispersive spectrometry and X-ray diffraction analyses of three coal dusts and examine their effects on rat lung alveolar macrophages (AM) in cell culture. The coal dusts were obtained from coal surfaces of anthracite, meager and fat coal mines. AM were harvested by bronchoalveolar lavage from adult male Wistar rats and were cultured in Eagle's medium at 37°C.



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1) Hurley, JF; Copland, L. ; Dodgson, , Jr; Jacobsen, M. ; Simple pneumoconiosis and exposure to dust at 10 British coal miners. Br J. Ind. Med. 42: 120127. 1982.
2) Attfield, M D; Seixas, NS. Prevalence of pneumoconiosis and its relationship to dust exposure in cohort of bituminous, U. S. coal miners and ex-miners. Am. J. Ind. Med. 27: 137151, 1995.
3) Lapp, NL, V., Castranova How silicosis and coal workers' pneumoconiosis develop- a cellular assessment. Occup. Med. 8: 3556, 1993.
4) Morgenstern, S., Flor, R., Kessler, G., B., Klein Automated determination of NAD-coupled enzymes. Determination of lactic dehydrogenase. Analytical Biochem. 13: 149161, 1965.
5) Bajpai R, Waseem M, Gupta GS, Kaw JL. Ranking toxicity of industrial dusts by bronchoalveolar lavage fluid analysis. Toxicology 73: 161-7, 1992.
6) Barm, PJ. Biological markers and occupational lung disease: mineral dust-induced respiratory disorders. Exp. Lung. Res. 20: 457-70, 1994.
7) Huang, X, Laurent PA, Zalma R, Pezerat H. Inactivation of alpha 1-antitrypsin by aqueous coal solution: possible relation to the emphysema of coal workers. Chem. Res. Toxical. 6: 452-8, 1993.
8) Huang, X, R., Zalma, H., Pezerat Factors that influence the formation and stability of hydrated ferrous sulfate in coal dusts. Possible relation to the emphysema of coal miners. Chem. Res. Toxical. 7: 451-7, 1994.

Composition of Coal Dusts and Their Cytotoxicity on Alveolar Macrophages

  • C. Y. Lee (a1), S.L Lee (a2), C. E. Sheehan (a1) and Yinghua Wang (a1)


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