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Neuropeptidase activities in plasma after acute restraint stress. Interaction with cortico-limbic areas

Published online by Cambridge University Press:  17 February 2016

Ana Belén Segarra ,
Joaquín Hernández ,
Isabel Prieto ,
Marc de Gasparo  and
Manuel Ramírez-Sánchez
Ana Belén Segarra
Affiliation:
Unit of Physiology, University of Jaén, Jaén, Spain
Joaquín Hernández
Affiliation:
Unit of Physiology, University of Jaén, Jaén, Spain
Isabel Prieto
Affiliation:
Unit of Physiology, University of Jaén, Jaén, Spain
Marc de Gasparo
Affiliation:
Cardiovascular and Metabolic Syndrome Adviser, Rossemaison, Switzerland
Manuel Ramírez-Sánchez*
Affiliation:
Unit of Physiology, University of Jaén, Jaén, Spain
*
Manuel Ramírez-Sánchez, Unit of Physiology, University of Jaén, Bldg B3-263, 23071 Jaén, Spain. Tel: +34 953 212302; Fax: +34 953 212943; E-mail: msanchez@ujaen.es
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Abstract

Objective

To evaluate the influence of acute restraint stress (ARS) on plasma enkephalinase and oxytocinase activities. ARS modifies basal activities in cortico-limbic regions of rats and induces changes in the correlations observed between these regions. The interactions between plasma and cortico-limbic activities will be also evaluated.

Methods

Enkephalinase (AlaAP and LeuAP) and oxytocinase (P-LeuAP) activities were fluorometrically determined in plasma of control and stressed rats using aminoacyl-β-naphthylamides (aaNNap), AlaNNap and LeuNNap as substrates.

Results

No differences in enzymatic activities were observed between control and stressed animals in plasma. In contrast, highly significant positive and negative correlations between plasma and cortico-limbic regions were demonstrated in controls. Stress conditions significantly alter the pattern of these correlations.

Conclusion

The present results clearly support a connection between plasma and brain involving certain neuropeptidase activities that change under stress conditions.

Keywords

cortico-limbic regionsenkephalinase activityoxytocinase activityplasmastress
Type
Short Communication
Information
Acta Neuropsychiatrica , Volume 28 , Supplement 4 , August 2016 , pp. 239 - 243
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Copyright
© Scandinavian College of Neuropsychopharmacology 2016 

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