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Methylphenidate treatment causes oxidative stress and alters energetic metabolism in an animal model of attention-deficit hyperactivity disorder

Published online by Cambridge University Press:  12 August 2013

Clarissa M. Comim ,
Karin M. Gomes ,
Gislaine Z. Réus ,
Fabrícia Petronilho ,
Gabriela K. Ferreira ,
Emílio L. Streck ,
Felipe Dal-Pizzol  and
João Quevedo
Clarissa M. Comim*
Affiliation:
Laboratory of Neurosciences, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Karin M. Gomes
Affiliation:
Laboratory of Neurosciences, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Gislaine Z. Réus
Affiliation:
Laboratory of Neurosciences, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Fabrícia Petronilho
Affiliation:
Laboratory of Experimental Pathophysiology, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Gabriela K. Ferreira
Affiliation:
Laboratory of Experimental Pathophysiology, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Emílio L. Streck
Affiliation:
Laboratory of Experimental Pathophysiology, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
Felipe Dal-Pizzol
Affiliation:
Laboratory of Experimental Pathophysiology, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
João Quevedo
Affiliation:
Laboratory of Neurosciences, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil
*
Prof. Clarissa M. Comim, PhD, Laboratory of Neuroscience, PPGCS, UNASAU, University of Southern Santa Catarina, 88806-000 Criciúma, SC, Brazil. Tel/Fax: +55 48 3431 2736; E-mail: clarissamc@terra.com.br
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Abstract

Objectives

To evaluate oxidative damage through the thiobarbituric acid-reactive species (TBARS) and protein carbonyl groups; antioxidant enzymatic system – superoxide dismutase (SOD) and catalase (CAT); and energetic metabolism in the brain of spontaneously hypertensive adult rats (SHR) after both acute and chronic treatment with methylphenidate hydrochloride (MPH).

Methods

Adult (60 days old) SHRs were treated during 28 days (chronic treatment), or 1 day (acute treatment). The rats received one i.p. injection per day of either saline or MPH (2 mg/kg). Two hours after the last injection, oxidative damage parameters and energetic metabolism in the cerebellum, prefrontal cortex, hippocampus, striatum and cortex were evaluated.

Results

We observed that both acute and/or chronic treatment increased TBARS and carbonyl groups, and decreased SOD and CAT activities in many of the brain structures evaluated. Regarding the energetic metabolism evaluation, the acute and chronic treatment altered the energetic metabolism in many of the brain structures evaluated.

Conclusion

We observed that both acute and chronic use of methylphenidate hydrochloride (MPH) in adult spontaneously hypertensive rats (SHRs) was associated with increased oxidative stress and energetic metabolism alterations. These data also reinforce the importance of the SHR animal model in further studies regarding MPH.

Keywords

energetic metabolismmethylphenidateoxidative stressSHR rats
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 26 , Issue 2 , April 2014 , pp. 96 - 103
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

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