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  • Print publication year: 2020
  • Online publication date: July 2020

2 - Perfusion Bioreactors:The Set-Up and Process Characterisation


In this chapter, we give an overview of the challenges and objectives in operating mammalian cell perfusion cultures and provide guidelines for the design and set-up of lab-scale bioreactor systems. Next, the control structure needed to maintain long-term stable and viable cultures is illustrated, followed by a section on the media design. In the last section, we discuss steady-state operation, reactor, and process dynamics, including product quality considerations. Comparisons with current technologies, particularly with respect to product quality, are also discussed.