Book contents
- Frontmatter
- Contents
- List of Abbreviations
- List of Symbols
- 1 Perfusion Mammalian Cell Culture for Recombinant Protein Manufacturing
- 2 Perfusion Bioreactors:The Set-Up and Process Characterisation
- 3 Scale-Down Models and Sensors for Process Development
- 4 Design and Optimisation of Mammalian Cell Perfusion Cultures
- 5 Clinical- and Commercial-Scale Reactors
- 6 Mechanistic and Statistical Modelling of Bioprocesses
- References
- Index
2 - Perfusion Bioreactors:The Set-Up and Process Characterisation
Published online by Cambridge University Press: 17 July 2020
Book contents
- Frontmatter
- Contents
- List of Abbreviations
- List of Symbols
- 1 Perfusion Mammalian Cell Culture for Recombinant Protein Manufacturing
- 2 Perfusion Bioreactors:The Set-Up and Process Characterisation
- 3 Scale-Down Models and Sensors for Process Development
- 4 Design and Optimisation of Mammalian Cell Perfusion Cultures
- 5 Clinical- and Commercial-Scale Reactors
- 6 Mechanistic and Statistical Modelling of Bioprocesses
- References
- Index
Summary
In this chapter, we give an overview of the challenges and objectives in operating mammalian cell perfusion cultures and provide guidelines for the design and set-up of lab-scale bioreactor systems. Next, the control structure needed to maintain long-term stable and viable cultures is illustrated, followed by a section on the media design. In the last section, we discuss steady-state operation, reactor, and process dynamics, including product quality considerations. Comparisons with current technologies, particularly with respect to product quality, are also discussed.
- Type
- Chapter
- Information
- Perfusion Cell Culture Processes for BiopharmaceuticalsProcess Development, Design, and Scale-up, pp. 20 - 45Publisher: Cambridge University PressPrint publication year: 2020