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Chapter 5 - The IVF Cycle to Come: Laboratory Innovations

Published online by Cambridge University Press:  02 March 2020

By
Denny Sakkas  and
David K Gardner
Edited by
Alice D. Domar ,
Denny Sakkas  and
Thomas L. Toth
Alice D. Domar
Affiliation:
Boston IVF, Waltham, MA
Denny Sakkas
Affiliation:
Boston IVF, Waltham, MA
Thomas L. Toth
Affiliation:
Boston IVF, Waltham, MA
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Summary

In the first reported case of IVF and embryo development in the human, Rock and Menkin made the following description of the culture and development of a fertilized embryo: “The eggs were incubated in serum for 22.5 hours, being washed in salt solution before and after incubation, and then exposed to a washed sperm suspension in Locke’s solution for two hours at room temperature. They were again washed in Locke’s solution and cultured in fresh serum for 45 hours.

Type
Chapter
Information
Patient-Centered Assisted Reproduction
How to Integrate Exceptional Care with Cutting-Edge Technology
 , pp. 54 - 66
Publisher: Cambridge University Press
Print publication year: 2020

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References

Menkin, MF, Rock, J In Vitro Fertilization and Cleavage of Human Ovarian Eggs. Am J Obstet Gynecol. 1948.Google Scholar
Rock, J, MM. In Vitro Fertilization and Cleavage of Human Ovarian Eggs. Science (80-). 1944; 100(2588): 105–7.Google Scholar
Ferrick, L, Lee, YSL, Gardner, DK. Reducing Time to Pregnancy and Facilitating the Birth of Healthy Children through Functional Analysis of Embryo Physiology. Biol Reprod. 2019.Google Scholar
Sati, L, Bennett, D, Janes, M, Huszar, G. Next Day Determination of Ejaculatory Sperm Motility After Overnight Shipment of Semen to Remote Locations. J Assist Reprod Genet. 2015; 32(1): 117–25.CrossRefGoogle ScholarPubMed
Kanakasabapathy, MK, Sadasivam, M, Singh, A, Preston, C, Thirumalaraju, P, Venkataraman, M, et al. An Automated Smartphone-Based Diagnostic Assay for Point-of-Care Semen Analysis. Sci Transl Med. 2017; 9(382).Google Scholar
Agarwal, et al. Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility. World J Men’s Health. 2019; 37(3): 296–312.CrossRefGoogle ScholarPubMed
Jenkins, TG, Aston, KI, James, ER, Carrell, DT. Sperm Epigenetics in the Study of Male Fertility, Offspring Health, and Potential Clinical Applications. Syst Biol Reprod Med. 2017; 63(2): 69–76.Google Scholar
Jodar, M, Sendler, E, Moskovtsev, SI, Librach, CL, Goodrich, R, Swanson, S, et al. Absence of Sperm RNA Elements Correlates with Idiopathic Male Infertility. Sci Transl Med. 2015; 7(295).CrossRefGoogle ScholarPubMed
Jodar, M, Sendler, E, Krawetz, SA. The Protein and Transcript Profiles of Human Semen. Cell Tissue Res. 2016; 363(1): 85–96.Google Scholar
Jodar, M, Selvaraju, S, Sendler, E, Diamond, MP, Krawetz, SA. The Presence, Role and Clinical Use of Spermatozoal RNAs. Hum Reprod Update. 2013; 19(6): 604–24.Google Scholar
Jenkins, TG, Aston, KI, Pflueger, C, Cairns, BR, Carrell, DT. Age-Associated Sperm DNA Methylation Alterations: Possible Implications in Offspring Disease Susceptibility. PLoS Genet. 2014; 10(7): e1004458.CrossRefGoogle ScholarPubMed
Carrell, DT, Aston, KI, Oliva, R, Emery, BR, De Jonge, CJ. The “Omics” of Human Male Infertility: Integrating Big Data in a Systems Biology Approach. Cell Tissue Res. 2016.Google Scholar
Khandwala, YS, Zhang, CA, Lu, Y, Eisenberg, ML. The Age of Fathers in the USA Is Rising: An Analysis of 168 867 480 Births from 1972 to 2015. Hum Reprod. 2017; 32(10): 2110–16.Google Scholar
Vaughan, DA, Sakkas, D. Sperm Selection Methods in the 21st Century. Biol Reprod.Google Scholar
de Wagenaar, B, Berendsen, JTW, Bomer, JG, Olthuis, W, van den Berg, A, Segerink, LI. Microfluidic Single Sperm Entrapment and Analysis. Lab Chip. 2015; 15(5): 1294–301.CrossRefGoogle ScholarPubMed
Smith, GD, Takayama, S. Application of Microfluidic Technologies to Human Assisted Reproduction. Mol Hum Reprod. 2017; gaw076.Google Scholar
Bhagwat, S, Sontakke, S, , KD, Parte, P, Jadhav, S. Chemotactic Behavior of Spermatozoa Captured Using a Microfluidic Chip. Biomicrofluidics. 2018; 12(2): 024112.Google Scholar
Ko, Y-J, Maeng, J-H, Hwang, SY, Ahn, Y. Design, Fabrication, and Testing of a Microfluidic Device for Thermotaxis and Chemotaxis Assays of Sperm. SLAS Technol. 2018; 23(6): 507–15.CrossRefGoogle ScholarPubMed
Eravuchira, PJ, Mirsky, SK, Barnea, I, Levi, M, Balberg, M, Shaked, NT. Individual Sperm Selection by Microfluidics Integrated with Interferometric Phase Microscopy. Methods. 2018; 136: 152–9.Google Scholar
Amaral, S, Da Costa, R, Wübbeling, F, Redmann, K, Schlatt, S. Raman Micro-Spectroscopy Analysis of Different Sperm Regions: A Species Comparison. MHR Basic Sci Reprod Med. 2018;24 (4): 185–202.Google Scholar
Weng, L, Lee, GY, Liu, J, Kapur, R, Toth, TL, Toner, M. On-Chip Oocyte Denudation from Cumulus–Oocyte Complexes for Assisted Reproductive Therapy. Lab Chip. 2018; 18(24): 3892–902.Google Scholar
Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Pregnancies After Intracytoplasmic Injection of Single Spermatozoon into an Oocyte. Lancet. 1992; 340(8810): 17–18.Google Scholar
Adamson, GD, de Mouzon, J, Chambers, GM, Zegers-Hochschild, F, Mansour, R, Ishihara, O, et al. International Committee for Monitoring Assisted Reproductive Technology: World Report on Assisted Reproductive Technology. Fertil Steril. 2011; 110(6): 1067–80.Google Scholar
Zhang, Z, Dai, C, Huang, JY, Wang, X, Liu, J, Ru, C, et al. Robotic Immobilization of Motile Sperm for Clinical Intracytoplasmic Sperm Injection. IEEE Trans Biomed Eng. 2018.Google Scholar
Lu, Z, Zhang, X, Leung, C, Esfandiari, N, Casper, RF, Sun, Y. Robotic ICSI (Intracytoplasmic Sperm Injection). IEEE Trans Biomed Eng 2011.Google Scholar
Han, C, Zhang, Q, Ma, R, Xie, L, Qiu, T, Wang, L, et al. Integration of Single Oocyte Trapping, in Vitro Fertilization and Embryo Culture in a Microwell-Structured Microfluidic Device. Lab Chip. 2010.Google Scholar
Kattera, S, Chen, C. Short Coincubation of Gametes in in Vitro Fertilization Improves Implantation and Pregnancy Rates: A Prospective, Randomized, Controlled Study. Fertil Steril. 2003; 80(4): 1017–21.Google Scholar
Bavister, BD. Culture of Preimplantation Embryos: Facts and Artifacts. Hum Reprod Update. 1995; 1(2): 91–148.Google Scholar
Gardner, DK, Lane, M. Culture of viable mammalian embryos. In: Cibelli, J, Lanza, R, Campbell, K, West, M (eds.), Principles of Cloning. San Diego: Academic Press. 2014; 63–84.CrossRefGoogle Scholar
Gardner, DK, Lane, M. Culture systems for the human embryo. In: Gardner, DK, Weissman, A, Howles, C, Shoham, Z (eds.) Textbook of Assisted Reproductive Techniques. Informa Healthcare. 2018; 200–24.Google Scholar
Pribenszky, C, Nilselid, A-M, Montag, M. Time-Lapse Culture with Morphokinetic Embryo Selection Improves Pregnancy and Live Birth Chances and Reduces Early Pregnancy Loss: A Meta-Analysis. Reprod Biomed Online. 2017; 35(5): 511–20.CrossRefGoogle ScholarPubMed
Paulson, RJ, Reichman, DE, Zaninovic, N, Goodman, LR, Racowsky, C. Time-Lapse Imaging: Clearly Useful to Both Laboratory Personnel and Patient Outcomes Versus Just Because We Can Doesn’t Mean We Should. Fertil Steril. 2018; 109(4): 584–91.Google Scholar
Armstrong, S, Bhide, P, Jordan, V, Pacey, A, Farquhar, C. Time-Lapse Systems for Embryo Incubation and Assessment in Assisted Reproduction. Cochrane database Syst Rev. 2018; 5:CD011320.Google Scholar
Wale, PL, Gardner, DK. The Effects of Chemical and Physical Factors on Mammalian Embryo Culture and Their Importance for the Practice of Assisted Human Reproduction. Hum Reprod Update. 2016; 22(1): 2–22.CrossRefGoogle ScholarPubMed
Gardner, DK, Kelley, RL. Impact of the IVF Laboratory Environment on Human Preimplantation Embryo Phenotype. J Dev Orig Health Dis. 2017; 8(4): 418–35.CrossRefGoogle ScholarPubMed
Bormann, C, Cabrera, L, Heo, YS, Takayama, S, Smith, G. Dynamic Microfluidic Embryo Culture Enhances Blastocyst Development of Murine and Bovine Embryos. Biol Reprod. 2017.Google Scholar
Heo, YS, Cabrera, LM, Bormann, CL, Shah, CT, Takayama, S, Smith, GD. Dynamic Microfunnel Culture Enhances Mouse Embryo Development and Pregnancy Rates. Hum Reprod. 2010.CrossRefGoogle Scholar
Gardner, DK, Pool, TB, Lane, M. Embryo Nutrition and Energy Metabolism and Its Relationship to Embryo Growth, Differentiation, and Viability. Semin Reprod Med. 2000.Google Scholar
Gardner, DK. Mammalian Embryo Culture in the Absence of Serum or Somatic Cell Support. Cell Biol Int.; 18(12): 1163–79.Google Scholar
Gardner, DK, Sakkas, D. Mouse Embryo Cleavage, Metabolism and Viability: Role of Medium Composition. Hum Reprod. 1993; 8(2).Google Scholar
Steeves, TE, Gardner, DK. Temporal and Differential Effects of Amino Acids on Bovine Embryo Development in Culture. Biol Reprod. 1999; 61(3): 731–40.Google Scholar
Lane, M, Gardner, DK. Differential Regulation of Mouse Embryo Development and Viability by Amino Acids. J Reprod Fertil. 1997; 109(1): 153–64.Google ScholarPubMed
Thouas, GA, Dominguez, F, Green, MP, Vilella, F, Simon, C, Gardner, DK. Soluble Ligands and Their Receptors in Human Embryo Development and Implantation. Endocrine Rev. 2015; 36(1): 92–130.CrossRefGoogle ScholarPubMed
Ferraz, MAMM, Rho, HS, Hemerich, D, Henning, HHW, van Tol HTA, Hölker, M, et al. An Oviduct-on-a-Chip Provides an Enhanced in Vitro Environment for Zygote Genome Reprogramming. Nat Commun. 2018.Google Scholar
Urbanski, JP, Johnson, MT, Craig, DD, Potter, DL, Gardner, DK, Thorsen, T. Noninvasive Metabolic Profiling Using Microfluidics for Analysis of Single Preimplantation Embryos. Anal Chem. 2008; 80(17): 6500–7.Google Scholar
Trounson, A, Mohr, L. Human Pregnancy Following Cryopreservation, Thawing and Transfer of an Eight-Cell Embryo. Nature. 1983.Google Scholar
Lane, M, Schoolcraft, WB, Gardner, DK. Vitrification of Mouse and Human Blastocysts Using a Novel Cryoloop Container-less Technique. Fertil Steril. 1999; 72(6): 1073–8.Google Scholar
Rienzi, L, Gracia, C, Maggiulli, R, LaBarbera, AR, Kaser, DJ, Ubaldi, FM, et al. Oocyte, Embryo and Blastocyst Cryopreservation in ART: Systematic Review and Meta-Analysis Comparing Slow-Freezing Versus Vitrification to Produce Evidence for the Development of Global Guidance. Hum Reprod Update. 2016.CrossRefGoogle Scholar
von Versen-Höynck, F, Schaub, AM, Chi, Y-Y, Chiu, K-H, Liu, J, Lingis, M, et al. Increased Preeclampsia Risk and Reduced Aortic Compliance With in Vitro Fertilization Cycles in the Absence of a Corpus Luteum. Hypertens. 1979; 73(3): 640–9.Google Scholar
Roy, TK, Brandi, S, Peura, TT. Chapter 20 Gavi-Automated Vitrification Instrument. Methods Mol Biol. 2017; 1568: 261–77.CrossRefGoogle ScholarPubMed
Arav, A, Natan, Y, Kalo, D, Komsky-Elbaz, A, Roth, Z, Levi-Setti, PE, et al. A New, Simple, Automatic Vitrification Device: Preliminary Results with Murine and Bovine Oocytes and Embryos. J Assist Reprod Genet. 2018; 35(7): 1161–8.Google Scholar
Pyne, DG, Liu, J, Abdelgawad, M, Sun, Y. Digital Microfluidic Processing of Mammalian Embryos for Vitrification. PLoS One. 2014; 9(9): e108128.Google Scholar
Gardner, DK, Balaban, B. Assessment of Human Embryo Development Using Morphological Criteria in an Era of Time-Lapse, Algorithms and “OMICS”: Is Looking Good Still Important? Mol Hum Reprod. 2016; 22(10): 704–18.Google Scholar
Gardner, DK, Meseguer, M, Rubio, C, Treff, NR. Diagnosis of Human Preimplantation Embryo Viability. Hum Reprod Update. 2015; 21(6): 727–47.CrossRefGoogle ScholarPubMed
Sanchez, T, Seidler, EA, Gardner, DK, Needleman, D, Sakkas, D. Will Noninvasive Methods Surpass Invasive for Assessing Gametes and Embryos? Fertil Steril. 2017; 108(5): 730–7.CrossRefGoogle ScholarPubMed
Gardner, DK, Reineck, P, Gibson, BC, Thompson, J. Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology. In: Agarwal, A, Varghese, A, Nagy, ZP (eds.) Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices. New Jersey: Humana Press. 2019.Google Scholar
Sanchez, T, Wang, T, Pedro, MV, Zhang, M, Esencan, E, Sakkas, D, et al. Metabolic Imaging with the Use of Fluorescence Lifetime Imaging Microscopy (FLIM) Accurately Detects Mitochondrial Dysfunction in Mouse Oocytes. Fertil Steril. 2018; 110(7): 1387–97.Google Scholar
Capalbo, A, Romanelli, V, Patassini, C, Poli, M, Girardi, L, Giancani, A, et al. Diagnostic Efficacy of Blastocoel Fluid and Spent Media as Sources of DNA for Preimplantation Genetic Testing in Standard Clinical Conditions. Fertil Steril. 2018; 110(5): 870–9.e5Google Scholar
Xu, J, Fang, R, Chen L, Chen D, Xiao J-P, Yang W, et al. Noninvasive Chromosome Screening of Human Embryos by Genome Sequencing of Embryo Culture Medium for in Vitro Fertilization. Proc Natl Acad Sci USA. 2016; 113(42): 11907–12.Google Scholar
Sutton-McDowall, ML, Gosnell, M, Anwer, AG, White, M, Purdey, M, Abell, AD, et al. Hyperspectral Microscopy Can Detect Metabolic Heterogeneity Within Bovine Post-Compaction Embryos Incubated under Two Oxygen Concentrations (7% Versus 20%). Hum Reprod. 2017; 32(10): 2016–25.Google Scholar
Sanchez, T, Wang, T, Pedro, MV, Zhang, M, Esencan, E, Sakkas, D, et al. Metabolic Imaging with the Use of Fluorescence Lifetime Imaging Microscopy (FLIM) Accurately Detects Mitochondrial Dysfunction in Mouse Oocytes. Fertil Steril. 2018; 110(7).Google Scholar
Liu, Y, Chapple, V, Feenan, K, Roberts, P, Matson, P. Time-Lapse Deselection Model for Human Day 3 in Vitro Fertilization Embryos: The Combination of Qualitative and Quantitative Measures of Embryo Growth. Fertil Steril. 2016; 105(3): 656–62.e1.Google Scholar
Petersen, BM, Boel, M, Montag, M, Gardner, DK. Development of a Generally Applicable Morphokinetic Algorithm Capable of Predicting the Implantation Potential of Embryos Transferred on Day 3. Hum Reprod. 2016; 31(10).Google Scholar
Khosravi, P, Kazemi, E, Zhan, Q, Malmsten, JE, Toschi, M, Zisimopoulos, P, et al. Deep Learning Enables Robust Assessment and Selection of Human Blastocysts After in Vitro Fertilization. Nat Digit Med. 2019; 2.Google Scholar
Tran, A, Cooke, S, Illingworth, PJ, Gardner, DK. A Study of the Diagnostic Accuracy of Deep Learning, a Novel Approach for Predicting the Fetal Heart Implantation Rate Following Time-Lapse Incubation. Hum Reprod. 2019; in press.Google Scholar
Sakkas, D, Brent Barrett, C, Alper, MM. Types and Frequency of Non-Conformances in an IVF Laboratory. Hum Reprod. 2018; 33(12).Google Scholar
Nagamatsu, G, Hayashi, K. Stem Cells, in Vitro Gametogenesis and Male Fertility. Reproduction. 2017; 154(6): F79–91.Google Scholar
Ma, H, Marti-Gutierrez, N, Park, S-W, Wu, J, Lee, Y, Suzuki, K, et al. Correction of a Pathogenic Gene Mutation in Human Embryos. Nature. 2017; 548(7668): 413–19.Google Scholar
Hayashi, K, Ogushi, S, Kurimoto, K, Shimamoto, S, Ohta, H, Saitou, M. Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice. Science (80-). 2012; 338(6109): 971–5.Google Scholar
Hayashi, K, Saitou, M. Generation of Eggs from Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells. Nat Protoc. 2013; 8(8): 1513–24.Google Scholar
Richani, D, Constance, K, Lien, S, Agapiou, D, Stocker, WA, Hedger, MP, et al. Cumulin and FSH Cooperate to Regulate Inhibin B and Activin B Production by Human Granulosa-Lutein Cells In Vitro. Endocrinology. 2019; 160(4): 853–62.CrossRefGoogle ScholarPubMed
Vuong, LN, Ho, VNA, Ho, TM, Dang, VQ, Phung, TH, Giang, NH, et al. Effectiveness and Safety of in Vitro Maturation of Oocytes Versus in Vitro Fertilisation in Women with High Antral Follicle Count: Study Protocol for a Randomised Controlled Trial. 2018; 8(12): e023413.Google Scholar
Treff, NR, Zimmerman, R, Bechor, E, Hsu, J, Rana, B, Jensen, J, et al. Validation of Concurrent Preimplantation Genetic Testing for Polygenic and Monogenic Disorders, Structural Rearrangements, and Whole and Segmental Chromosome Aneuploidy with a Single Universal Platform. Eur J Med Genet. 2019.CrossRefGoogle Scholar

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