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  • Print publication year: 2004
  • Online publication date: May 2010

32 - Late reproductive sequelae of treatment for childhood cancer

from Part III - Management of specific disorders



Tremendous therapeutic advances in the management of childhood malignancies mean that the majority of children can realistically hope for long-term survival. Cancer in childhood is rare, with about 1400 new cases per year in the UK and a cumulative risk of 1 in 650 by the age of 15 years (Stiller, 1997; Wallace, 1997). With survival rates in excess of 70%, it is estimated that by the year 2010, 1 in 250 of the young adult population will be a long-term survivor of childhood cancer (Fig. 32.1) (Bleyer, 1990). However, the successful treatment of cancer in childhood with chemotherapy and or radiotherapy is associated with a number of unwanted side effects in later life (Wallace et al., 2001). Therefore, the major challenge faced by paediatric oncologists today is to sustain the excellent survival rates while striving to improve the quality of life of the survivors.

One of the most important issues for the survivors of childhood cancer is the impact of their disease and its treatment on reproductive function and the implications for the health of their offspring. In the female, chemotherapy and radiotherapy may damage the ovary and hasten oocyte depletion, resulting in loss of hormone production, truncated fecundity and a premature menopause (Meirow, 2000; Thomson et al., 2002). Infertility is one of the most commonly encountered and psychologically traumatic late complications of treatment for childhood cancer, and consequently, strategies to preserve fertile potential are being pursued.

Abir, R, Franks, S, Mobberley, M A, Moore, P A, Margara, R A, Winston, R M (1997). Mechanical isolation and in vitro growth of preantral and small antral human follicles. Fertil Steril 68, 682–688
Abir, R, Fisch, B, Raz, A, Nitke, S, Ben-Rafael, Z (1998). Preservation of fertility in women undergoing chemotherapy: current approach and future prospects. J Assist Reprod Genet 15, 469–478
Ahmed Eddiary, N A, Lenton, E A, Cooke, I D (1994). Hypothalamic—pituitary ageing: progressive increase in FSH and LH concentrations throughout the reproductive life in regularly menstruating women. Clin Endocrinol 41, 199–206
Ataya, K, Ramahi-Ataya, A (1993). Reproductive performance of female rats treated with cyclophosphamide and/or LHRH agonist. Reprod Toxicol 7, 229–235
Ataya, K, Rao, L V, Lawrence, E, Kimmel, R (1995a). Luteinizing hormone-releasing hormone agonist inhibits cyclophosphamide-induced ovarian follicle depletion in rhesus monkeys. Biol Reprod 52, 365–372
Ataya, K, Pydyn, E, Ramahi-Ataya, A, Orton, C G (1995b). Is radiation-induced ovarian failure in rhesus monkeys preventable by leuteinizing hormone-releasing hormone agonist? Preliminary observations. J Clin Oncol Endocrinol Metab 80, 790–795
Atkinson, H G, Apperley, J F, Dawson, K, Goldman, J M, Winston, R M (1994). Successful pregnancy after embryo cryopreservation after BMT for CML. Lancet 344, 199
Baird, D T, Webb, R, Campbell, B K, Harkness, L M, Gosden, R G (1999). Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at —196℃C. Endocrinology 140, 462–471
Balow, J E (1993). Risk for sustained amenorrhoea in patients with systemic lupus erythematosus receiving intermittent pulse cyclophosphamide therapy. Ann Intern Med 1, 366–369
Bath, L E, Critchley, H O, Chambers, S E, Anderson, R A, Kelnar, C J, Wallace, W H (1999). Ovarian and uterine characteristics after total body irradiation in childhood and adolescence: response to sex steroid replacement. Br J Obstet Gynaecol 106, 1265–1272
Bath, L E, Anderson, R A, Critchley, H O D, Kelnar, C J H, Wallace, W H B (2001). Hypothalamic—pituitary—ovarian dysfunction after prepubertal chemotherapy and cranial irradiation for acute leukaemia. Hum Reprod 16, 1838–1844
Bines, J, Oleske, D M, Cobleigh, M A (1996). Ovarian function in premenopausal women treated with adjuvant chemotherapy for breast cancer. J Clin Oncol 14, 1718–1729
Birkebaek, N H, Fisker, S, Clausen, N, Tuovinen, V, Sindet-Pedersen, S, Christiansen, J S (1998). Growth and endocrinological disorders up to 21 years after treatment for acute lymphoblastic leukaemia in childhood. Med Pediatr Oncol 30, 351–356
Bleyer, W A (1990). The impact of childhood cancer on the United States and the world. Cancer 40, 355–367
Block, E (1952). Quantitative morphological investigations of the follicular system in women. Variations at different ages. Acta Anat 14, 108–123
Block, E (1953). A quantitative morphological investigation of the follicular system in newborn female infants. Acta Anat 17, 201–206
Blumenfeld, Z (2000). Ovarian rescue/protection from chemotherapeutic agents (1). J Soc Gynecol Invest 8, S60–S64
Blumenfeld, Z, Avivi, I, Linn, S, Epelbaum, R, Ben-Shahar, M, Haim, N (1996). Prevention of irreversible chemotherapy-induced ovarian damage in young women with lymphoma by a gonadotrophin-releasing hormone agonist in parallel to chemotherapy. Hum Reprod 11, 1620–1626
Blumenfeld, Z, Shapiro, D, Shteinberg, M, Avivi, I, Nahir, M (2000). Preservation of fertility and ovarian function and minimizing gonadotoxicity in young women with systemic lupus erythematosus treated by chemotherapy. Lupus 9, 401–405
Bosma, G C, Custer, R P, Bosma, M J (1983). A severe combined deficiency mutation in the mouse. Nature 301, 527–530
Boumpas, D T, Austin, H A III, Vaughan, E M, Yarboro, C H, Klippel, J H, Balow J E (1993). Risk for sustained amenorrhoea in patients with systemic lupus erythematosus receiving intermittent pulse cyclophosphamide therapy. Ann Intern Med 1, 366–369
Brennan, B M, Rahim, A, Mackie, E M, Eden, O B, Shalet, S M (1998). Growth hormone status in adults treated for acute lymphoblastic leukaemia in childhood. Clin Endocrinol 48, 777–783
Brook, P F, Radford, J A, Shalet, S M, Joyce, A D, Gosden, R G (2001). Isolation of germ cells from human testicular tissue for low temperature storage and autotransplantation. Fertil Steril 75, 269–274
Brusamolino, E, Lunghi, F, Orlandi, E et al. (2000). Treatment of early-stage Hodgkin's disease with four cycles of ABVD followed by adjuvant radiotherapy:analysis and efficacy and long-term toxicity. Haematologica 85, 103–105
Byrne, J, Fears, T R, Gail, M H et al. (1992). Early menopause in long-term survivors of cancer during adolescence. Am J Obstet Gynecol 166, 788–793
Cain, L, Chatterjee, S, Collins, T J (1995). In vitro folliculogenesis of rat preantral follicles. Endocrinology 136, 3369–3377
Candy, C J, Wood, M J, Whittingham, D G (1995). Follicular development in cryopreserved marmoset ovarian tissue after transplantation. Hum Reprod 10, 2334–2338
Candy, C J, Wood, M J, Whittingham, D G (2000). Restoration of normal reproductive life span after grafting of cryopreserved ovaries. Hum Reprod 15, 1300–1304
Carroll, J, Gosden, R G (1993). Transplantation of frozen-thawed mouse primordial follicles. Hum Reprod 8, 1163–1167
Chatterjee, R, Goldstone, A H (1996). Gonadal damage and effects on fertility in adult patients with haematological malignancy undergoing stem cell transplantation. Bone Marrow Transplant 17, 159–167
Chapman, A M, Marrett, L D, Darlington, G (1979). Cytotoxic-induced ovarian failure in Hodgkin's disease. I. Hormone function. J Am Med Assoc 242, 1877–1881
Chapman, R M, Sutcliffe, S B, Malpas, J S (1981). Cytotoxic-induced ovarian failure in women treated with MOPP chemotherapy for Hodgkin's disease. Am J Med 71, 552–556
Chiarelli, A M, Marrett, L D, Darlington, G (1999). Early menopause and infertility in females after treatment for childhood cancer diagnosed in 1964–1988 in Ontario, Canada. Am J Epidemiol 150, 245–254
Clark, S T, Radford, J A, Crowther, D, Swindell, R, Shalet, S M (1995). Cytotoxic induced ovarian failure in women treated for Hodgkin's disease: a comparative study of MVPP and a seven-drug hybrid regimen. J Clin Oncol 13, 134–139
Cox, S L, Shaw, J M, Jenkin, G (1996). Transplantation of cryopreserved fetal ovarian tissue to adult recipients in mice. J Reprod Fertil 107, 315–322
Critchley, H O, Buckley, C H, Anderson, D C (1990). Experience with a ‘physiological’ steroid replacement regimen for the establishment of a receptive endometrium in women with premature ovarian failure. Br J Obstet Gynaecol 97, 804–810
Critchley, H O, Wallace, W H, Shalet, S M, Mamtora, H, Higginson, J, Anderson, D C (1992). Abdominal irradiation in childhood; the potential for pregnancy. Br J Obstet Gynaecol 99, 392–394
Critchley, H O, Wang, H, Kelly, R W, Gebbie, A E, Glasier, A F (1998). Progestin receptor isoforms and prostaglandin dehydrogenase in the endometrium of women using a levonorgestrel-releasing intrauterine system. Hum Reprod 13, 1210–1217
Crowne, E C, Moore, C, Wallace, W H B et al. (1992). A novel variant of growth hormone insufficiency following low dose cranial irradiation. Clin Endocrinol 36, 59–68
Eppig, J J, O'Brien, M (1996). Development in vitro of mouse oocytes from primordial follicles. Biol Reprod 54, 197–207
Eppig, D G, Schroeder, A C (1989). Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol Reprod 41, 268–276
Faddy, M J, Gosden, R G (1996). A model conforming the decline in follicle numbers to the age of menopause in women. Hum Reprod 11, 1484–1486
Faddy, M J, Gosden, R G, Edwards, R (1983). Ovarian follicle dynamics in mice: a comparative study of three inbred strains and an F1 hybrid. J Endocrinol 96, 23–33
Faddy, M J, Gosden, R G, Gougeon, A, Richardson, S J, Nelson, J F (1992). Accelerated disappearance of ovarian follicles in mid-life: implications for forecasting menopause. Hum Reprod 7, 1342–1346
Familiari, G, Caggiati, A, Nottola, S A, Ermini, M, Di Benedetto, M R, Motta, P M (1993). Ultrastructure of human ovarian primordial follicles after combination chemotherapy for Hodgkin's disease. Hum Reprod 8, 2080–2087
Fisher, B, Cheung, A Y (1984). Delayed effect of radiation therapy with or without chemotherapy on ovarian function in women with Hodgkin's disease. Acta Radiol Oncol 23, 43–48
Gosden, R G (1990). Restitution of fertility in sterilised mice by transferring primordial ovarian follicles. Hum Reprod 5, 117–122
Gosden, R G, Baird, D T, Wade, J C, Webb, R (1994a). Restoration of fertility to oopherectomized sheep by ovarian autografts stored at -196℃C. Hum Reprod 9, 597–603
Gosden, R G, Boulton, M I, Grant, K, Webb, R (1994b). Follicular development from ovarian xenografts in SCID mice. J Reprod Fertil 101, 619–623
Gosden, R G, Wade, J C, Fraser, H M, Sandow, J, Faddy, M J (1997a). Impact of congenital and experimental hypogonadism on the radiation sensitivity of the mouse ovary. Hum Reprod 12, 2483–2488
Gosden, R G, Rutherford, A J, Norfolk, D R (1997b). Ovarian banking for cancer patients: transmission of malignant cells in ovarian grafts. Hum Reprod 12, 403
Green, D M, Hall, B, Zevon, A (1989). Pregnancy outcome after treatment for acute lymphoblastic leukaemia during childhood or adolescence. Cancer 64, 235–244
Green, S H, Smith, A U, Zuckerman, S (1956). The number of oocytes in ovarian autografts after freezing and thawing. J Endocrinol 13, 333–334
Greenwald, G S, Moor, R M (1989). Isolation and preliminary characterization of pig primordial follicles. J Reprod Fertil 87, 561–571
Grundy, R, Gosden, R G, Hewitt, M et al. (2001a). Fertility preservation for children treated for cancer (1): scientific advances and research dilemmas. Arch Dis Child 84, 355–359
Grundy, R, Larcher, V, Gosden, R G et al. (2001b). Personal practice: fertility preservation for children treated for cancer (2); ethics of consent for gamete storage and experimentation. Arch Dis Child 84, 360–362
Guanasena, K T, Villines, P M, Crister, E S, Crister, J K (1997). Live births with autologous transplant of cryopreserved mouse ovaries. Hum Reprod 12, 101–116
Hawkins, M M (1994). Pregnancy outcome and offspring after childhood cancer. Br Med J 309, 1034–1040
Hawkins, M M, Smith, R A (1989). Pregnancy outcomes in childhood cancer survivors; probable effect of abdominal irradiation. Int J Cancer 43, 399–402
Hawkins, M M, Draper, G J, Smith, R A (1989). Cancer among 1348 survivors of childhood cancer. Int J Cancer 43, 975–978
Holm, K, Laursen, E M, Brocks, V, Muller, J (1995). Pubertal maturation of the internal genitalia: an ultrasound evaluation of 166 healthy girls. Ultrasound Obstet Gynecol 6, 155–181
Hovatta, O, Silye, R, Kraustz, T et al. (1996). Cryopreservation of human ovarian tissue by using dimethylsulphoxide and propandiol-sucrose as cryoprotectants. Hum Reprod 11, 1268–1272
Hudson, M M, Greenwald, C, Thompson, E et al. (1993). Efficacy and toxicity of multi agent chemotherapy and low-dose involved-field radiotherapy in children and adolescents with Hodgkin's disease. J Clin Oncol 11, 100–108
Kim, S S, Gosden, R G, Radford, J A et al. (1999). A model to test the safety of human ovarian tissue transplantation after cryopreservation: xenografts of ovarian tissue from cancer patients into NOD/LtSz-scid mice. [Annual Meeting of the American Society of Reproductive Medicine, Toronto, Canada.] Fertil Steril Suppl p. 3
Larsen, E C, Loft, A, Holm, K, Muller, J, Brocks, V, Anderson, A N (2000). Oocyte donation in women cured of cancer with bone marrow transplantation including total body irradiation in adolescence. Hum Reprod 15, 1505–1508
Leporrier, M, Theobald, P, Roffe, J L, Muller, G (1987). A new technique to protect ovarian function in young women undergoing pelvic irradiation. Heterotopic ovarian autotransplantation. Cancer 60, 2201–2204
Li, F P, Fine, W, Jaffe, N, Holmes, G E, Holmes, F F (1979). Offspring of patients treated for cancer in childhood. J Natl Cancer Inst 62, 1193–1197
Li, F P, Gimbrere, K, Gelber, R D et al. (1987). Outcome of pregnancy in survival of Wilm's tumour. J Am Med Assoc 257, 216–219
Littley, M D, Shalet, S M, Beardwell, C G, Ahmed, S R, Applegate, G, Sutton, M L (1989). Hypopituitarism following external radiotherapy for pituitary tumours in adults. Q J Med 70, 145–160
Livesey, E A, Brook, C G D (1988). Gonadal dysfunction after treatment of intracranial tumours. Arch Dis Child 63, 495–500
Louis, L, Lemaryi, L R, Best, W R (1956). Treatment of chronic granulocytic leukaemia with myleran. Arch Int Med 97, 299–308
Ludwig, M, Al-Hasani, S, Felderbaum, R, Diedrich, K (1999). New aspects of cryopreservation of oocytes and embryos in assisted reproduction and future perspectives. Hum Reprod 14, 162–185
Lushbaugh, C C, Casarett, G W (1976). The effects of gonadal irradiation in clinical radiation therapy: a review. Cancer 37, 1111–1125
Mackie, E J, Radford, M, Shalet, S M (1996). Gonadal function following chemotherapy for childhood Hodgkin's disease. Med Pediatr Oncol 27, 74–78
Matsumoto, M, Shinohara, O, Ishiguro, H et al. (1999). Ovarian function after bone marrow transplantation performed before menarche. Arch Dis Child 80, 452–454
Marcello, M F, Nuciforo, G, Romeo, R et al. (1990). Structural and ultrastructural study of the ovary in childhood leukaemia after successful treatment. Cancer 66, 2099–2104
Meirow, D (1999).Ovarian injury and modern options to preserve fertility in female cancer patients treated with high-dose radio-chemotherapy for haemato-oncological neoplasias and other cancers. Leuk Lymphoma 33, 65–76
Meirow, D (2000). Reproduction post chemotherapy in young cancer patients. Mol Cell Endocrinol 169, 123–131
Meirow, D, Nugent, D, Epstein, M et al. (1998). An in vitro study of the effects of chemotherapy on human primordial follicles. Hum ReprodA13
Mok, C C, Lau, C S, Wong, R W (1998). Risk factors for ovarian failure in patients with systemic lupus erythematosus receiving cyclophosphamide therapy. Arthritis Rheum 41, 831–837
Montz, F J, Wolff, A J, Gambone, J C (1991). Gonadal protection and fecundity rates in cyclophosphamide-treated rats. Cancer Res 51, 2124–2126
Moore, H C (2000). Following treatment for breast cancer. The incidence of treatment related amenorrhoea is related to patient age and to the treatment regimen. Curr Oncol Rep 2, 587–593
Morita, Y, Perez, G I, Paris, F et al. (2000). Oocyte apoptosis is suppressed by disruption of the acid sphingomyelinase gene or by sphingosine-1-phosphate therapy. Nat Med 6, 1109–1114
Nicholson, H S, Byrne, J (1993). Fertility and pregnancy after treatment for cancer during childhood or adolescence. Cancer 71, 3392–3399
Nugent, D, Meirow, D, Brook, P F, Aubard Y, Gosden R G (1997). Transplantation in reproductive medicine: previous experience, present knowledge and future prospects. Hum Reprod Update 3, 267–280
Nygaard, R, Clausen, N, Simes, M A et al. (1991). Reproduction following treatment for childhood leukaemia: a population based prospective cohort study of fertility and offspring. Med Pediatr Oncol 19, 459–466
Oktay, K (2001). Ovarian tissue cryopreservation and transplantation: preliminary findings and implications for cancer patients. Hum Reprod Update 7, 526–534
Oktay, K, Karlikaya, G (2000). Ovarian function after transplantation of frozen, banked, autologous ovarian tissue. N Engl J Med 342, 1919
Oktay, K, Nugent, D, Newton, H, Salha, O, Chatterjee, P, Gosden, R G (1997). Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 67, 481–486
Oktay, K, Newton, H, Mullan, J, Gosden, R G (1998). Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Hum Reprod 13, 1133–1138
Oktay, K, Karlikaya, G G, Aydin, B A (2000a). Ovarian cryopreservation and transplantation: basic aspects. Mol Cell Endocrinol 169, 105–108
Oktay, K, Newton, H, Gosden, R G (2000b). Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice. Fertil Steril 73, 599–603
Oktay, K, Economos, K, Kan, M, Rucinski, J, Veeck, L, Rosenwaks, Z (2001). Endocrine function and oocyte retrieval after autologous transplantation of ovarian cortical strips to the forearm. J Am Med Assoc 286, 1490–1493
Parkes, A S, Smith, A U (1952). Regeneration of rat ovarian tissue grafted after exposure to low temperatures. Proc Roy Soc Lond 140, 455–467
Parrot, D M (1960). The fertility of mice with orthotopic grafts derived from frozen tissue. J Reprod Fertil 1, 230–241
Pasqualini, T, Escobar, M E, Domene, H, Muriel, F S, Pavlovsky, S, Rivarola, M A (1987). Evaluation of gonadal function following long-term treatment for acute lymphoblastic leukaemia in girls. Am J Pediatr Hematol Oncol 9, 15–22
Perez, G I, Knudson, C M, Leykin, L, Korsmeyer, S J, Tilly, J L (1997). Apoptosis-associated signalling pathways are required for chemotherapy-mediated female germ cell destruction. Nat Med 3, 1228–1232
Perez, G I, Maravei, D V, Trbovich, A M, Cidlowski, J A, Tilly, J L, Hughes, F M Jr (2000). Identification of the potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells. Biol Reprod 63, 1358–1369
Porcu, E, Fabbri, R, Seracchioli, R, Ciotti, P M, Magrini, O, Flamigni, C (1997). Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril 68, 724–726
Radford, J A, Leibermann, B A, Brison, D R et al. (2001). Orthotopic reimplantation of cryopreserved ovarian cortical strips after high-dose chemotherapy for Hodgkin's lymphoma. Lancet 357, 1172–1175
Richardson, S J, Senikas, V, Nelson, J F (1987). Follicular depletion during the menopausal transition: evidence for accelerated loss and ultimate exhaustion. J Clin Endocrinol Metab 65, 1231–1237
Robertson, C M, Hawkins, M M, Kingston, J E (1994). Late deaths and survival after children with cancer: implications for cure. Br Med J 309, 162–166
Roy, S K, Greenwald, G S (1989). Hormonal requirements for the growth and differentiation of hamster preantral follicles in long-term culture. J Reprod Fertil 87, 103–114
Roy, S K, Treacy, B J (1993). Isolation and long-term culture of human preantral follicles. Fertil Steril 59, 783–790
Royal College of Obstetricians and Gynaecologists (2000). Storage of Ovarian and Prepubertal Testicular Tissue: Report of a Working Party. Royal College of Obstetricians and Gynaecologists, London
Salooja, N, Szydlo, R M, Socie, G et al. (2001). Pregnancy outcomes after peripheral blood or bone marrow transplantation: a retrospective survey. Lancet 358, 271–276
Sanders, J E (1991). The impact of marrow transplant preparation regimens on subsequent growth and development. Semin Hematol 28, 244–249
Sanders, J E, Hawley, J, Levy, W et al. (1996). Pregnancies following high-dose cyclophosphamide with or without high-dose busulfan or total-body irradiation and bone marrow transplantation. Blood 87, 3045–3052
Sanger, W G, Olson, J H, Sherman, J K (1992). Semen cryobanking for men with cancer: criteria change. Fertil Steril 58, 1024–1027
Schimmer, A D, Quatermain, M, Imrie, K et al. (1998). Ovarian function after bone marrow transplantation. J Clin Oncol 16, 2359–2363
Shaw, J M, Bowles, J, Koopman, P, Wood, E C, Trounson A O (1996). Fresh and cryopreserved ovarian tissue samples from donors with lymphoma transmit the cancer to graft recipients. Hum Reprod 11, 1668–1673
Shaw, J M, Cox, S L, Trounson, A O, Jenkin, G (2000). Evaluation of the long-term function of cryopreserved ovarian grafts in the mouse, implications for human applications. Mol Cell Endocrinol 161, 103–110
Sherman, B M, West, J H, Korenman, S G (1976). The menopausal transition: analysis of LH, FSH, estradiol, and progesterone concentrations during menstrual cycles of older women. J Clin Endocrinol Metab 42, 629–636
Siris, E S, Leventhal, B G, Vaitukaitis, J L (1976). Effects of childhood leukaemia and chemotherapy on puberty and reproductive function in girls. N Engl J Med 294, 1143–1146
Snijders, M P, Goeij, A F, Debets-Te, Baerts M J, Rousch, M J, Koudstaal, J, Bosman, F T (1992). Immunocytochemical analysis of oestrogen receptors and progesterone receptors in the human uterus throughout the menstrual cycle and after the menopause. J Reprod Fertil 94, 363–371
Spears, N, Boland, N I, Murray, A A, Gosden, R G (1994). Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Hum Reprod 9, 527–532
Stiller C A (1997). Aetiology and epidemiology. In Paediatric Oncology: Clinical Practice and Controversies, 2nd edn, Pinkerton C R, Plowman P N, eds., pp. 3–21. Chapman & Hall, London
SyOrtin, T T, Shostak, C A, Donaldson, S S (1990). Gonadal status and reproductive function following treatment for Hodgkin's disease in childhood: the Stanford experience. Int J Radiat Oncol, Biol, Phys 304, 1377–1382
Sztein, J M, O'Brie, M J, Farley, J S, Mobraaten, L (1998). Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. Biol Reprod 58, 1071–1074
Teinturier, C, Hartmann, O, Valteau-Couanet, D, Benhamou, E, Bougneres, P F (1998). Ovarian function after autologous bone marrow transplantation in childhood: high dose busulfan is a major cause of ovarian failure. Bone Marrow Transplant 22, 989–994
Thibaud, E, Rodriguez-Macias, K, Trivin, C, Esperou, H, Michon, J, Brauner, R (1998). Ovarian function after bone marrow transplantation. Bone Marrow Transplant 21, 287–290
Thomas, P R, Winstanly, D, Peckham, M J, Austin, D E, Murray, M A, Jacobs, H S (1976). Reproductive and endocrine function in patients with Hodgkin's disease: effects of oopheropexy and irradiation. Br J Cancer 33, 226–231
Thomson, A B, Critchley, H O D, Kelnar, C J H, Wallace, W H B (2002). Late reproductive sequelae following treatment of childhood cancer and options for fertility preservation. Adult sequelae of childhood endocrine disorders. Baillière's Best Pract Res Clin Endocrinol Metab 16, 311–334
Torrance, C, Telfer, E, Gosden, R G (1989). Quantitative study of the development of isolated mouse pre-antral follicles in collagen gel culture. J Reprod Fertil 87, 367–374
Wallace W H B (1997). Growth and endocrine function following the treatment of childhood malignant disease. In Paediatric Oncology: Clinical Practice and Controversies, 2nd edn, Pinkerton C R, Plowman P N, eds., pp. 706–731. Chapman & Hall, London
Wallace, W H B, Walker, D A (2001). Conference consensus statement: ethical and research dilemmas for fertility preservation in children treated for cancer. Hum Fertil 4, 69–76
Wallace, W H, Shalet, S M, Crowne, E C, Morris-Jones, P H, Gattamaneni, H R (1989a). Ovarian failure following abdominal irradiation in childhood: natural history and prognosis. Clin Oncol 1, 75–79
Wallace, W H, Shalet, S M, Hendry, J H, Morris-Jones, P H, Gattamaneni, H R (1989b). Ovarian failure following abdominal irradiation in childhood: The radiosensitivity of the human oocyte. Br J Radiol 62, 995–998
Wallace, W H B, Shalet, S M, Crowne, E C, Morris-Jones, P H, Gattamaneni, H R, Price, D A (1989c). Gonadal dysfunction due to cis-platinum. Med Pediatr Oncol 17, 409–413
Wallace, W H, Shalet, S M, Tetlow, L J, Morris-Jones, P H (1993). Ovarian function following the treatment of childhood acute lymphoblastic leukaemia. Med Pediatr Oncol 21, 333–339
Wallace, W H B, Blacklay, A, Eiser, C et al. (2001). Developing strategies for long term follow up of survivors of childhood cancer. Br Med J 323, 271–274
Wallace, W H B, Thomson, A B, Kelsey, T W (2003). The radiosensitivity of the human oocyte. Hum Reprod 18, 117–121
Waxman, J H X, Terry, Y A, Wrigley, P F M et al. (1982). Gonadal function in Hodgkin's disease: long-term follow-up of chemotherapy. Br Med J 285, 1612–1613
Weissman, A, Gotlieb, L, Colgan, T, Jurisicova, A, Greenblatt, E M, Casper, R F (1999). Preliminary experience with subcutaneous human ovarian cortex transplantation in the NOD-SCID mouse. Biol Reprod 60, 1462–1467
Wells, S A Jr, Ellis, G J, Gunnells, J C, Schneider, A B, Sherwood, L M (1976). Parathyroid autotransplantation in parathyroid hyperplasia. N Engl J Med 295, 57–62
Whitehead, E, Shalet, S M, Blackledge, G, Todd, I, Crowther, D, Beardwell, C G (1983). The effect of combination chemotherapy on ovarian function in women treated for Hodgkin's disease. Cancer 52, 988–993