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Methods in Molecular Biophysics
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Book description

Our knowledge of biological macromolecules and their interactions is based on the application of physical methods, ranging from classical thermodynamics to recently developed techniques for the detection and manipulation of single molecules. These methods, which include mass spectrometry, hydrodynamics, microscopy, diffraction and crystallography, electron microscopy, molecular dynamics simulations, and nuclear magnetic resonance, are complementary; each has its specific advantages and limitations. Organised by method, this textbook provides descriptions and examples of applications for the key physical methods in modern biology. It is an invaluable resource for undergraduate and graduate students of molecular biophysics in science and medical schools, as well as research scientists looking for an introduction to techniques beyond their specialty. As appropriate for this interdisciplinary field, the book includes short asides to explain physics aspects to biologists and biology aspects to physicists.

Reviews

'… a valuable contribution to the field. … There is nothing quite like it at the moment.'

Sir Tom Blundell FRS - University of Cambridge

'… one of the most comprehensive and highly relevant texts on biophysics that I have encountered in the last 10 years, clearly written and up-to-date … a must-have for biophysicists working in all lines of research …'

Nikolaus Grigorieff - Brandeis University

'… a wonderful up-to-date treatise on the many and diverse methods used … in the fields of molecular biophysics, physical biochemistry, molecular biology, biological physics and the new and emerging field of quantum nanobiology.'

Karl J. Jalkanen - Quantum Protein Centre, Technological University of Denmark

'… a valuable resource for novice and seasoned biophysicists alike.'

Dan Minor - California Institute for Quantitative Biomedical Research, University of California

'… the book I consult first when faced with an unfamiliar experimental technique. Both classic analytical techniques and the latest single-molecule methods appear in this single comprehensive reference.'

Philip Nelson - University of Pennsylvania and author of Biological Physics (2004)

'… valuable both for students and research scientists.'

Michael G. Rossmann - Hanley Professor of Biological Sciences, Purdue University

'A great achievement … awaits the student who reads this book … an excellent reference for the seasoned practitioner of biophysical chemistry.'

Milton H Werner - The Rockefeller University

'This well written, thorough, and elegantly illustrated book provides the connections between molecular biophysics and biology that every aspiring young biologist needs.'

Stephen H. White - University of California at Irvine

'… I enthusiastically recommend Methods in Molecular Biophysics to anyone who wishes to know more about the techniques by which the properties of biological macromolecules are determined.'

David Worcester - University of Missouri

'A book that teaches the methods well, creates the intellectual framework of our understanding, and can guide the field. Earlier efforts by Cohn and Edsall, Tanford, Edsall and Wyman, and Cantor and Schimmel have served this important purpose in the past, but the advance of time and technology has diluted the force of these classic works in contemporary Biophysics, both in the teaching and the practices of the field. How welcome, then, a clearly written, thoughtful and modern text that will serve well, both in formal courses and as a reference. The authors have built each method from its fundamental premises and principles, have successfully covered an impressive span of topics, and will be rewarded by attention from an audience that hungers for the next defining text in Molecular Biophysics.'

D. M. Engelman - Yale University, New Haven,

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