Skip to main content Accessibility help
×
Hostname: page-component-7c8c6479df-94d59 Total loading time: 0 Render date: 2024-03-29T01:27:42.325Z Has data issue: false hasContentIssue false

Chapter 28 - Oocyte and embryo cryopreservation

Published online by Cambridge University Press:  16 May 2011

David K. Gardner
Affiliation:
University of Melbourne
Botros R. M. B. Rizk
Affiliation:
University of South Alabama
Tommaso Falcone
Affiliation:
Cleveland Clinic Foundation
Get access

Summary

This chapter focuses on vitrification technologies for cryopreservation in human assisted reproductive technology (ART). For cryopreservation of human embryos, PROH and dimethylsulfoxide (DMSO) have been used as the dominant cryoprotective agents (CPAs). There are several protocols that have been introduced for human day 2-3 vitrification. The differences between the protocols are related to the type and concentration of CPAs and duration of exposure of CPAs. The chapter presents a summary of those protocols and clinical outcomes. A large blastocoel might lessen cryopreservative potential due to ice crystal formation during the rapid cooling phase of vitrification. To overcome this problem, shrinkage of the blastocoel was thought to be the appropriate approach. Cryopreservation of human oocytes has been significantly improved by refined slow-freezing methods and new vitrification techniques. In future, vitrification will become the most suitable method for cryopreservation of any cells and tissues.
Type
Chapter
Information
Human Assisted Reproductive Technology
Future Trends in Laboratory and Clinical Practice
, pp. 313 - 325
Publisher: Cambridge University Press
Print publication year: 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×