For cancer patients of childbearing age, fertility restoration often becomes an important concern after disease remission. Transplantation has so far been the only option to re-establish ovarian function from cryopreserved ovarian tissue in cancer survivors. Apart from the interaction between granulosa cells (GCs) and oocytes, follicles require neighboring stromal cells to support their growth. Grafting isolated follicles would allow not only the introduction of a high and known number of follicles into the host, but also assessment of follicular quality before grafting. As in case of a natural ovary, the main goal of an artificial ovary is to offer an environment that allows folliculogenesis to occur. In order to assemble an artificial ovary, the scaffold should act as a vehicle to graft isolated follicles, ensuring preservation of their 3D structure. This chapter provides a comprehensive description of the assembly of an artificial ovary and the involvement of tissue engineering strategies.