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Chapter 9 - Sperm Preparation

Published online by Cambridge University Press:  16 February 2022

David Mortimer
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
University of Dundee
José Antonio Castilla
HU Virgen de las Nieves, Granada
Roelof Menkveld
Stellenbosch University, South Africa
Ulrik Kvist
Karolinska Institutet, Stockholm
Juan G. Alvarez
Centro ANDROGEN, La Coruña
Trine B. Haugen
Oslo Metropolitan University
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The physiology and basic principles of sperm washing and other preparative techniques are described, including the limitations and even dangers posed by some methods. Optimized methods for sperm preparation using direct swim-up from semen and density gradient centrifugation are provided as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on standardization and robustness, and minimizing the risk iatrogenic damage to the spermatozoa and avoiding errors. There are sections on selecting which method to use, dealing with atypical semen specimens, processing multiple specimens, and potential new technology for preparing human spermatozoa for use in assisted conception treatment.

Publisher: Cambridge University Press
Print publication year: 2022

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