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Chapter 10 - Sperm Cryopreservation

Published online by Cambridge University Press:  16 February 2022

David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

The basic princples of cryobiology are described for both slow freezing and vitrification of spermatozoa. Specific aspects of cryopreserving human spermatozoa are discussed in detail, incluidng the formulation of cryopreservation media and their proper use. Alternative packaging devices are discussed in relation to the achievement of correct cooling and warming curves as well as effective biocontainment. High security straws are recommended as the best method to use from both perspectives, and a standard operating procedure (SOP) for easy use at the bench is provided. SOPs for human sperm vitrification techniques are also gven. Quality control and risk management aspects of sperm freezing and for cryobank organzation are described. Finally, there is a section on sperm donation.

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Publisher: Cambridge University Press
Print publication year: 2022

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